QUANTITATIVE MASS SPECTROMETRY ANALYSIS REVEALS SIMILAR SUBSTRATE CONSENSUS MOTIF FOR HUMAN MPS1 KINASE AND PLK1.

BACKGROUND: Members of the Mps1 kinase family play an essential and evolutionarily conserved role in the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis.Human Mps1 (hMps1) is highly phosphorylated during mitosis and many phosphorylation sites have been identified.However, the u

The here study of tumourigenesis commonly involves the use of established cell lines or single cell suspensions of primary tumours.Standard methods for the generation of short-term tumour cell cultures include the disintegration of tissue based on enzymatic and mechanical stress.Here, we describe a simple and rapid method for the preparation of sin